Isolation, Optimization and Characterization of Lipase Producing Bacteria from Abbatoir Soil

Microorganisms can easily degrade agro-industrial wastes in a solid-state fermentation process thereby synthesizing many important commercial bio compounds including lipolytic enzymes. Screening different habitats for bacteria with lipase activity and optimizing its parameters facilities effective production usage industries. In this study, soil samples from Abattoirs Kaduna metropolis were screened presence of positive strains culture medium such as carbon source, inducers, nitrogen pH, temperature, inoculums level incubation period varied the purpose optimization. Maximum was recorded at 48hours incubation, occurred media containing groundnut peel rice husk, supplemented ammonium nitrate urea which are best sources. The isolated identified Pseudomonas biochemically, production, partial purification characterization determination molecular weight analysis. partially purified up to saturation using sulphate precipitation spectrophotometric assay used enzyme characterization. Lipases produced by pseudomonas have been widely pharmaceutical, food, pulp, textile, paper, leather industries among others due their stability, selectivity broad substrate specificity.